Dr.austin Ochieng Aluoch
Dr.austin Ochieng Aluoch
Doctor of Philosophy in Analytical Chemistry(SUNY-Binghamton, Binghamton), Masters of Science in Chemistry(University of Nairobi), November,Bachelors of Science in Chemistry(University of Nairobi).
Areas of Specialization:
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Publications (12 Records)
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Stability indicating ion-pair HPLC method for the determination of risedronate in a commercial formulation - 2004
Aluoch, A., Tatini, R., Parsons, D. M., Sadik, O. A. A simple, rapid, and reproducible analytical procedure has been developed for the assay of risedronate in pharmaceutical dosage forms. The method is based on ion-pair liquid chromatography with UV detection. 2 Separation is performed on an Eclipse XDB C-18 (4.6 x 150 mm(2), 3.5 mum particles) column, using 5 mM tetrabutylammonium phosphate as counter-ion in the mobile phase. The proposed method was extensively validated according to ICH guidelines for the assay determination. A linear relationship was found in the concentration range studied from 50 to 150 mug risedronate sodium per 25 muL injection. The method precision was below 1.2% relative standard deviation (RSD) (n = 9). The mean recovery of risedronate from commercial tablets was found to be in the range of 99.3-100.6%. The limit of detection (LOD) and limit of quantification (LOQ) of risedronate were found to be 30 and 100 ng, respectively. Since the method is stability indicating, it is also well suited for shelf-life studies of risedronate pharmaceutical preparations.
Journal of Liquid Chromatography and Related Technologies, 27(17), 2799-2813 (2004) Read More..
Bioactive Compounds from some Kenyan Ethnomedicinal Plants:
Myrsinaceae, Polygonaceae and Psiadia punctulata, - 2002
J. O. Midiwo, A. Yenesew, B. F. Juma, S. Dereses, J. A. Ayoo, A. Aluoch and S. Guchu There are several described medicinal plants in Kenya from a flora of approximately 10,000 members. Strong cross-medical information from the 42 ethnic groups points to the high potential of some of these species. The Myrsinaceae are well established ethno-anthelmintics and anti-bacterials. They are harbingers of long alkyl side chain benzoquinones which clearly have a protective function from their histochemical disposition. The main benzoquinone in the sub-family Myrsinodae is embelin while for the Maesodae it is maesaquinone together with its 5-acetyl derivative; the distribution of these benzoquinones by their alkyl side chain length or the presence/absence of a 6-methyl group is in accord with morphological sub-family de-limitation. The benzoquinones showed anti-feedant, anti-microbial, phytotoxic, acaricidal, insecticidal and nematicidal activity. Many other benzoquinones of medium and minor concentration were also isolated and characterised. Some plants belonging to the Polygonaceae which are widely used as ethno-anthelmintics have been studied. The common anthelmintic anthraquinones were obtained from all five Rumex species while the naphthalenic acetogenin derivative, nepodin was more selectively distributed. The leaf of Polygonum senegalense is up to 17% surface exudate; about thirteen non polar flavonoid derivatives (chalcones, dihydrochalcones, flavanones and a flavone) have been isolated from it. From the internal aerial tissues of this plant, the major flavonoids were common flavonoids, quercetin, kaempferol, luteolin and their glycosides. The only unique compound isolated from this plant was 2prime-glucosyl-6prime-hydroxy-4prime-methoxydihydrochalcone whose aglycone, uvangolatin is part of the exudate mixture. Other leaf exudate plants studied include the stomach-ache medicine, Psiadia punctulata (Compositae) from which novel methylated flavonoids, kaurene and trachyloban diterpenes have been found
Phytochemistry Reviews, 1,311-323 (2002) Read More..
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